Enzymes and Inhibitors
ABclonal Technology Circle Ligase
Circle Ligase is a thermostable ATP-dependent ligase that can catalyze the self-circularization of single-stranded DNA (ssDNA)/single-stranded RNA (ssRNA) strands simultaneously possessing a 5-phosphate group and a 3-hydroxyl group in the absence of complementary sequences, with the length of single-stranded DNA (ssDNA)/single-stranded RNA (ssRNA) being greater than 15bp.
ABclonal Technology Exonuclease III (E. coli)
Exonuclease III (E.coli) catalyzes the stepwise removal of mononucleotides from 3?-hydroxyl termini of duplex DNA. The preferred substrates are dsDNA with blunt or recessed 3?-, termini although the enzyme also acts at nicks in dsDNA to produce single-strand gaps. dsDNA with 3?-overhang are resistant to cleavage; when overhangs length is ≥ 4 bases，dsDNA are able to fully prevent ExoIII cleavage process. Exonuclease III has also been reported to have RNase H, 3?-phosphatase and AP-endonuclease activities.
Sino Biological Peptide Substrates: p38 Substrate
The synthetic peptide (IPTTPITTTYFFFKKK) contains serine/threonine protein kinase phosphorylation sites and is routinely evaluated as a substrate for p38 family kinases.
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa >=2,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 2KU
Phosphatase, Alkaline from bovine intestinal mucosa >=2,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 2KU
ABclonal Technology Endonuclease IV
Endonuclease IV derived from E. coli recognizes the depurine/depyrimidine (AP) site on the double- stranded DNA molecule, and cleaves the first phosphodiester bond at the 5 ?end of the AP site to form 3-OH and 5dRP . In addition, Endonuclease IV has 3?diesterase activity, which releases phosphoglyceraldehyde, intact deoxyribose 5 -phosphate, and phosphoric acid from the 3 ?end of DNA. The best substrate for this enzyme is AP double-stranded DNA, but it is also active against AP single-stranded DNA.
Sigma Aldrich Fine Chemicals Biosciences ADENOSINE DEAMINASE (ADA) 2ML
Adenosine Deaminase (ADA) from calf intestine 2mL
Sigma Aldrich Fine Chemicals Biosciences Phospholipase C from Clostridium perfringens (C. welchii) Type I, lyophilized powder, 10-50 units/mg protein | 9001-86-9 | MFCD00131895 | 125UN
Phospholipase C from Clostridium perfringens (C. welchii) Type I, lyophilized powder, 10-50 units/mg protein | 9001-86-9 | MFCD00131895 | 125UN
Sigma Aldrich Fine Chemicals Biosciences Phosphoglucose Isomerase from rabbit muscle Type XI, lyophilized powder, >=200 units/mg protein | 9001-41-6 | MFCD00131887 | 1KU
Phosphoglucose Isomerase from rabbit muscle Type XI, lyophilized powder, >=200 units/mg protein | 9001-41-6 | MFCD00131887 | 1KU
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa | 9001-78-9 | MFCD00131849 | 10g
Phosphatase, Alkaline from bovine intestinal mucosa | Mol Wt: dimer -160 kDa | 9001-78-9 | MFCD00131849 | 10g
Sigma Aldrich Fine Chemicals Biosciences Proteinase from Aspergillus melleus Type XXIII, >=3 units/mg solid | 9001-92-7 | MFCD00132092 | 25G
Proteinase from Aspergillus melleus Type XXIII, >=3 units/mg solid | 9001-92-7 | MFCD00132092 | 25G